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Agri Life NITROFIX TM - Av is a biological fertilizer based on a selected strain of naturally-occurring beneficial diazotrophic bacteria - Azotobacter vinelendii (NCIM -2821 , ATCC -12837 ). Owing to their unique metabolism they can actively fix atmospheric nitrogen in soil by conversion to ammonia. It is used as an effective soil application product.

Overdependence and abuse of nitrogenous fertilizers based on fossil fuel / chemical has resulted in depletion of beneficial soil microbial organisms. Cost of chemical nitrogenus fertilizers is increasing progressively and making it unaffordable to farmers. Biological nitrogen Fixation ( BNF) is proven concept for over a century and is a cost effective proposition.

Agri Life NITROFIX TM - AV contains cysts of Azotobacter vinelendii . It is formulated as carrier based powder with CFU count of 5 X 10 ^ 7 / g. and as 1*10^8 CFU/ml Liquid. Agri Life NITROFIX TM - AV is registered under the Fertilizer Control Order- Govt of India. Agri Life NITROFIX TM - AV is approved for use in Organic Agriculture.

Mode of Action
Nitrogen cannot be absorbed by the plants owing to the strong triple bonds between the nitrogen atoms making it inert and therefore non assimable by plants.

Enzyme production : Azotobacter vinelendii produces enzymes such as molybdenum-containing nitrogenase enzyme and two alternative nitrogenases; one in which vanadium replaces molybdenum and a second which contains neither transition metal but only iron.

Nitrogen fixation needs a large amount of energy in the form of ATP and an anaerobic environment (no oxygen environment). Thus Azotobacter develops a special defence mechanism against oxygen by intensifying its metabolism that reduces the oxygen concentration in its cells. It can also synthesise a protective 2Fe-2S protein which can bind to nitrogenase in conditions of oxygen stress to form an oxygen-stable complex that is inactive but protected from damage. Homocitrate ions play some role in the processes of Biological Nitrogen Fixation ( BNF) .

Nitrogenase action : This is an important enzyme used to convert atmospheric nitrogen into ammonium / nitrate ions which get fixed in soil and can be absorbed by the plants. The basic type is molybdenum-iron nitrogenase.

Bio Active compound synthesis : Azotobacter vinelendii synthesizes some biologically active substances including some phytohormones and vitamins thereby stimulating plant growth. They also facilitate the mobility of heavy metals in the soil and thus enhance bioremediation of soil from heavy metals, such as cadmium, mercury and lead.

Method of Application
Seed Treatment: Mix 10 g. of Agri Life NITROFIX TM - AV with 10 g. of crude sugar in sufficient water to make a slurry and coat 1 kg of seeds, dry in shade and sow / broadcast / dibble in the field
Note: Do not store treated / coated seeds more than 24 hrs
Seedling treatment: Mix 100 g. of Agri Life NITROFIX TM - AV with sufficient quantity of water and organic manure to form a slurry. The seedlings are dipped in this slurry for 30 minutes prior to planting so that the bacteria get attached to the roots.
Soil application: Mix 3-5 Kg/ acre of Agri Life NITROFIX TM - AV with compost and apply to an acre of soil.
Drip Irrigation: Mix 3 Kg / 3 L of Agri Life NITROFIX TM in drip stream.

Target Nutrition
Nitrogen supplementation

Agri Life NITROFIX TM - AV is suitable for application on Cereals , Millets , Pulses, Oilseeds, Fibre Crops , Sugar Crops , Forage Crops , Plantation crops ,Vegetables, Fruits, Spices , Flowers , Medicinal crops , Aromatic Crops , Orchards and Ornamentals.

Shelf Life
Agri Life NITROFIX TM - AV is stable for a period of 12 months from the date of manufacturing.

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Agri Life
SOM Phytopharma (India) Limited

154/A5, SVCIE, IDA Bollaram 502325
Medak Dist. (Hyderabad) AP, INDIA.