| Formulations | Carrier powder | Soluble powder | Soluble liquid |
| Potency | 5 X 10 ^ 7 /g | 1x 10 ^ 9 CFu/g | 1x 10 ^ 8 CFU/ml |
| Application Type | Seed dressing, soil application | Seed dressing, soil application, Foliar application | Seed dressing, soil application, Foliar application |
| Indian Fertilizer Regulatory (FCO) | Organic Certification – NPOP India | National Bio Diversity Board Compliance – India | Access benefit sharing agreement (ABS) – Nagoya protocol |
| Yes | Yes | Yes | Yes |
| Maximum Residue Limit (MRL) | Pre-Harvest Interval (PHI) | Re-entry barrier | Safe to predators, parasites and pollinators |
| Not applicable | 0 Days | 0 Days | Yes |
AgriLife Nitrofix®-AC contains cysts of Azotobacter chroococum. The composition is customised to comply with regulatory guidelines of respective countries. Azotobacter chroococum is a proprietary strain and compliant with National Bio Diversity Authority guidelines and has Access Benefit Sharing (ABS) agreement with the State Bio Diversity Board for payment of royalty to the habitat from where the microbes have been isolated. The strain is under Patent deposit certificate with Budapest Treaty recognised depository.
Nitrogen cannot be absorbed by the plants owing to the strong triple bonds between the nitrogen atoms making it inert and therefore non assimable by plants.
Enzyme production: Azotobacter chroococum produces enzymes such as ferredoxin, hydrogenase and nitrogenase
Nitrogen fixation needs a large amount of energy in the form of Adenosine Tri-Phosphate (ATP) and an anaerobic environment (no oxygen environment). Thus Azotobacter chroococum develops a special defence mechanism against oxygen by intensifying its metabolism that reduces the oxygen concentration in its cells. A special protein sheath is present that protects the important nitrogenase enzyme. Homocitrate ions play some role in the processes of Biological Nitrogen Fixation (BNF).
Nitrogenase action: This is an important enzyme used to convert atmospheric nitrogen into ammonium / nitrate ions which get fixed in soil and can be absorbed by the plants. The basic type is molybdenum-iron nitrogenase.
Bio active compound synthesis: Azotobacter chroococum synthesizes some biologically active substances including some phytohormones such as auxins thereby stimulating plant growth. They also facilitate the mobility of heavy metals in the soil and thus enhance bioremediation of soil from heavy metals, such as cadmium, mercury and lead.
AgriLife Nitrofix®-AC is available in the following formulations:
The following table gives details on Application methods and doses for the different formulations
| Carrier powder | Soluble powder | Soluble liquid | |
| Potency | 5x10^7 CFU/g | 1x10^9 CFU/g | 1x10^8 CFU/ml |
| Seed dressing | 1 Kg seed: 10g AgriLife Nitrofix®-AC + 10 g crude sugar | 1 Kg seed: 1g AgriLife Nitrofix®-AC + 10 g crude sugar | 1 Kg seed: 10ml AgriLife Nitrofix®-AC + 10 g crude sugar |
| Soil application(Soil drench OR Drip irrigation) | 1 Acre dose: 3-5Kg 1 Ha dose: 7.5- 12.5Kg |
1 Acre dose:100g 1 Ha dose: 250g |
1 Acre dose:1L 1 Ha dose: 2.5L |
| Soil application (Soil drench OR Drip irrigation) for Long duration crops / Orchards / Perennials | 1 Acre dose: 3-5Kg 1 Ha dose: 7.5- 12.5Kg Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose: 100g 1 Ha dose: 250g Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose:1L 1 Ha dose: 2.5L Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
| Foliar application | Not applicable | 1 Acre dose: 100g 1 Ha dose: 250g |
1 Acre dose:1L 1 Ha dose: 2.5L |
| Foliar application for Long duration crops / Orchards / Perennials | Not applicable | 1 Acre dose: 100g 1 Ha dose: 250g Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose:1L 1 Ha dose: 2.5L Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
Mix AgriLife Nitrofix®-AC with crude sugar in sufficient water to make a slurry and coat seeds and dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds more than 24 hrs
1. Mix at recommended doses with compost and apply at early life stages of crop along with other biofertilizers
2. Mix AgriLife Nitrofix®-AC at recommended doses in sufficient water and drench soil at early leaf stage/ 2-4 leaf stage / early crop life cycle
3. Drip Irrigation: If there are insoluble particles, filter the solution and add to drip tank.
4. Long duration crops / Perennial / Orchard crops: Dissolve AgriLife Nitrofix®-AC at recommended doses in sufficient water and apply as a drenching spray near root zone twice a year. It is recommended to have first application before the onset of the main monsoon / rainfall/ spring season and second application after the main monsoon / rainfall / autumn/ fall season.
Foliar application:
Mix AgriLife Nitrofix®-AC at recommended doses in sufficient water and spray on foliage. Apply twice a year for long duration crops. It is recommended to have first application before the onset of the main monsoon / rainfall/ spring season and second application after the main monsoon / rainfall / autumn/ fall season.
Note:
Do not store AgriLife Nitrofix®-AC solution for more than 24 hours after mixing in water.
A fraction of the product may not be soluble in water. It is advisable to filter and discard undissolved residue and use filtered liquid for spraying or feeding through drip irrigation.
Compatible with biofertilizers, organic fertilizers, organic manures, biopesticides, vermicompost, sea kelp (seaweed fertilizer), protein hydrolysates, organic carbon and rock phosphate
Note:
It is advisable to have a one-week gap if soil application pesticides are to be used after application of AgriLife Nitrofix®-AC.
Store in ambient temperature, not more than 35 degrees Celsius. Preferably store between 4 – 10-degree Celsius. Keep container closed after use. Store in a dry place.
6 months from the date of manufacturing.
AgriLife Nitrofix®-AC composition can be customized to meet local regulatory guidelines.
AgriLife Nitrofix®-AC is offered in India - as Nitrogen fixing bacteria – Biofertilizer under Fertilizer Control Order (FCO) of Govt of India with label claim of Azotobacter chroococum with 5*10^7 CFU/g in carrier-based powder formulation.
AgriLife Nitrofix®-AC is also offered as Soluble Powder formulation (1x 10 ^9 CFu/g) and Soluble Liquid formulation (1x 10 ^ 8 CFU/ml).
