| Formulations | Carrier powder | Soluble powder | Soluble liquid |
| Potency | 5 X 10 ^ 7 /g | 1x 10 ^9 CFu/g | 1x 10 ^ 8 CFU/ml |
| Application Type | Seed dressing, Soil application | Seed dressing, Soil application | Seed dressing, Soil application |
| Indian Fertilizer Regulatory (FCO) | Organic Certification – NPOP India | National Bio Diversity Board Compliance – India | Access benefit sharing agreement (ABS) – Nagoya protocol |
| Yes | Yes | Yes | Yes |
| Maximum Residue Limit (MRL) | Pre-Harvest Interval (PHI) | Re-entry barrier | Safe to predators, parasites and pollinators |
| Not applicable | 0 Days | 0 Days | Yes |
AgriLife Nitrofix®-GD contains vegetative cells of Gluconacetobacter diazotrophicus. The composition is customised to comply with regulatory guidelines of respective countries. Gluconacetobacter diazotrophicus is a proprietary strain and compliant with National Bio Diversity Authority guidelines and has Access Benefit Sharing (ABS) agreement with the State Bio Diversity Board for payment of royalty to the habitat from where the microbes have been isolated. The strain is under Patent deposit certificate with Budapest Treaty recognised depository.
Nitrogen cannot be absorbed by the plants owing to the strong triple bonds between the nitrogen atoms making it inert and therefore non assimable by plants.
Gluconacetobacter diazotrophicus is capable of fixing nitrogen in the roots, stems and leaves of sugarcane plant.Gluconacetobacter diazotrophicususes carbon source and it favours higher nitrogen fixation.
Gluconocetobacter diazotrophicus is an anaerobic tolerant diazotroph in which oxygen is used for the generation of large quantities of Adenosine Tri Phosphate (ATP) required for nitrogen fixation. Nitrate influences nitrogenase activity in Gluconocetobacter diazotrophicus at higher concentration.
It also produces acid where it solubilizes insoluble phosphates in broth assays.
Gluconocetobacter diazotrophicus primarly inhabits intercellular apoplastic spaces, the xylem, the xylem parenchyma and intracellularly without nodulation within the host cells. Within the stem, the bacterium enters at breaks caused by the separation of plantlets into individuals. Within the leaves, the bacterium enters through damaged stomata.
Bio Active compound synthesis: Gluconocetobacter diazotrophicus produces growth promoting substances such as indole -3-acetic acid (IAA), and gibberellins that promote root proliferation and increase the rootlet density and root branching which resulting in increased uptake of mineral and water which promotes cane growth and sugar recovery from the cane.
AgriLife Nitrofix®-GD is available in the following formulations:
The following table gives details on Application methods and doses for the different formulations
| Carrier powder | Soluble powder | Soluble liquid | |
| Potency | 5x10^7 CFU/g | 1x10^9 CFU/g | 1x10^8 CFU/ml |
| Seed dressing | 1 Kg seed: 10g AgriLife Nitrofix®-GD + 10 g crude sugar | 1 Kg seed: 1g AgriLife Nitrofix®-GD + 10 g crude sugar | 1 Kg seed: 10ml AgriLife Nitrofix®-GD + 10 g crude sugar |
| Soil application(Soil drench OR Drip irrigation) | 1 Acre dose: 3-5Kg 1 Ha dose: 7.5- 12.5Kg |
1 Acre dose:100g 1 Ha dose: 250g |
1 Acre dose:1L 1 Ha dose: 2.5L |
| Soil application (Soil drench OR Drip irrigation) for Long duration crops / Orchards / Perennials | 1 Acre dose: 3-5Kg 1 Ha dose: 7.5- 12.5Kg Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose: 100 g 1 Ha dose: 250g Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose:1L 1 Ha dose: 2.5L Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
| Foliar application | Not applicable | Not applicable | Not applicable |
Mix AgriLife Nitrofix®-GD with crude sugar in sufficient water to make a slurry and coat seeds and dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds more than 24 hrs
1. Mix at recommended doses with compost and apply at early life stages of crop along with other biofertilizers
2. Mix AgriLife Nitrofix®-GD at recommended doses in sufficient water and drench soil at early leaf stage/ 2-4 leaf stage / early crop life cycle
3. Drip Irrigation: If there are insoluble particles, filter the solution and add to drip tank.
4. Long duration crops / Perennial / Orchard crops: Dissolve AgriLife Nitrofix®-GD at recommended doses in sufficient water and apply as a drenching spray near root zone twice a year. It is recommended to have first application before the onset of the main monsoon / rainfall/ spring season and second application after the main monsoon / rainfall / autumn/ fall season.
Note:
Do not store AgriLife Nitrofix®-GD solution for more than 24 hours after mixing in water.
A fraction of the product may not be soluble in water. It is advisable to filter and discard undissolved residue and use filtered liquid for spraying or feeding through drip irrigation.
Compatible with biofertilizers, organic fertilizers, organic manures, biopesticides, vermicompost, sea kelp (seaweed fertilizer), protein hydrolysates, organic carbon and rock phosphate
Note:
It is advisable to have a one-week gap if soil application pesticides are to be used after application of AgriLife Nitrofix®-GD.
Store in ambient temperature, not more than 35 degrees Celsius. Preferably store between 4 – 10-degree Celsius. Keep container closed after use. Store in a dry place.
6 months from the date of manufacturing.
AgriLife Nitrofix®-GD composition can be customized to meet local regulatory guidelines.
AgriLife Nitrofix®-GD is also offered as Soluble Powder formulation (1x 10 ^ 9 CFu/g) and Soluble Liquid formulation (1x 10 ^ 8 CFU/ml).
