| Formulations | Wettable powder | Soluble powder |
| Potency | 1 X 10 ^ 8 CFu/ g | 1x 10 ^9 CFu/g |
| Application Type | Seed dressing, soil application, Foliar application | Seed dressing, soil application, Foliar application |
| Indian Pesticides Regulatory (CIB) | Organic Certification – NPOP India | National Bio Diversity Board Compliance – India | Access benefit sharing agreement (ABS) – Nagoya protocol |
| Under registration | Yes | Yes | Yes |
| Maximum Residue Limit (MRL) | Pre-Harvest Interval (PHI) | Re-entry barrier | Safe to predators, parasites and pollinators |
| Not applicable | 0 Days | 0 Days | Yes |
Biotilis™ contains endospores of Bacillus subtilis. The composition is customised to comply with regulatory guidelines of respective countries. Bacillus subtilis is a proprietary strain and compliant with National Bio Diversity Authority guidelines and has Access Benefit Sharing (ABS) agreement with the State Bio Diversity Board for payment of royalty to the habitat from where the microbes have been isolated. The strain is under Patent deposit certificate with Budapest Treaty recognised depository.
Substrate Competition for space and Nutrients: Biotilis™ colonizes maximum space and absorbs maximum nutrients available at the target site and thereby controls the pathogens by starving them for food and competing for space- Domino effect.
Mycoparasitism: Biotilis™ is a biological fungicide that attacks disease causing pathogens before they reach the plant system. It derives nutrition from the target pathogen and Biotilis™ spores multiply inside it and eventually kill the pathogen.
Enzyme production: Biotilis™ secretes certain antagonistic secondary metabolites which have an anti-fungal effect that pathogenise and kill the disease-causing organism. The metabolites released also have a Plant growth promoting effect which stimulates the rhizosphere for better root establishment and root health.
Biotilis™ is available in the following formulations:
The following table gives details on Application methods and doses for the different formulations
| Wettable powder | Soluble powder | |
| Potency | 1x10^8 CFU/g | 1x10^9 CFU/g |
| Seed dressing | 1 Kg seed: 10g Biotilis™ + 10g crude sugar | 1 Kg seed: 1g Biotilis™ + 10 g crude sugar |
| Soil application(Soil drench OR Drip irrigation) | 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 Kg |
1 Acre dose: 1 Kg 1 Ha dose: 2.5 Kg |
| Soil application (Soil drench OR Drip irrigation) for Long duration crops / Orchards / Perennials | 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 Kg Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose: 1 Kg 1 Ha dose: 2.5 Kg Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
| Foliar application | 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 Kg |
1 Acre dose: 1 Kg 1 Ha dose: 2.5 Kg |
| Foliar application for Long duration crops / Orchards / Perennials | 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 Kg Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
1 Acre dose: 1 Kg 1 Ha dose: 2.5 Kg Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. |
Seed dressing: Mix Biotilis™ with crude sugar in sufficient water to make a slurry and coat seeds and dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds more than 24 hrs
Soil application: Biotilis™ can be used as under:
Foliar application:
1. Foliar application to be done at early disease incidence. 1-2 follow up sprays to be done at weekly intervals
2. Mix Biotilis™ at recommended doses in sufficient water and spray on foliage/ fruit/plant. Apply twice a year for long duration crops. It is recommended to have first application before the onset of the main monsoon / rainfall/ spring season and second application after the main monsoon / rainfall / autumn/ fall season.
Note:
Do not store Biotilis™ solution for more than 24 hours after mixing in water.
A fraction of the product may not be soluble in water. It is advisable to filter and discard undissolved residue and use filtered liquid for spraying or feeding through drip irrigation.
Compatible with other BioPesticides , biofertilizers, organic fertilizers, organic manures, biopesticides, vermicompost, sea kelp (seaweed fertilizer), protein hydrolysates, organic carbon and rock phosphate.
Note:
It is advisable to have a one-week gap if soil application pesticides are to be used after application of Biotilis™
Store in ambient temperature, not more than 35 degrees Celsius. Preferably store between 4 – 10-degree Celsius. Keep container closed after use. Store in a dry place.
Biotilis™ has a shelf life of 2 year from the date of manufacturing.
Biotilis™ composition can be customized to meet local regulatory guidelines.
Biotilis™ is offered in India - as Bacillus subtilis with label claim of Bacillus subtilis with 1*10^8 CFU/g in wettable powder formulation
Biotilis™ is also offered as Soluble Powder formulation (1x 10 ^9 CFu/g) and as a concentrated product (1x 10 ^10 CFu/g)
