| Formulations | Soluble powder |
| Application Type | Seed dressing |
| Indian Fertilizer Regulatory (FCO) | Organic Certification – NPOP India | National Bio Diversity Board Compliance – India | Access benefit sharing agreement (ABS) – Nagoya protocol |
| Not applicable | Yes | Yes | Yes |
| Maximum Residue Limit (MRL) | Pre-Harvest Interval (PHI) | Re-entry barrier | Safe to predators, parasites and pollinators |
| Not applicable | 0 Days | 0 Days | Yes |
AgriLife Rootambio® comprises of Biofertilizers, PGPR, Nutrients, Prebiotics and Vitamins. The composition is customized to comply with regulatory guidelines of respective countries. All the microbes in AgriLife Rootambio® are proprietary strains and compliant with National Bio Diversity Authority guidelines and have Access Benefit Sharing (ABS) agreement with the State Bio Diversity Board for payment of royalty to the habitat from where the microbes have been isolated.
The strains are under Patent deposit certificate with Budapest Treaty recognised depository.
Biofertilizers:
• Nitrogen fixing bacteria – Paenibacillus durus ( Paenibacillus azotofixans)
• Phosphorous solubilizing bacteria - Bacillus megaterium
• Potassium mobilizing bacteria - Bacillus mucilaginosus
• Mycorrhiza - Rhizophagus irregularis
PGPR:
• Silica solubilizing bacteria – Bacillus mycoides
• Root enhancing bacteria - Bacillus spp
• Biomass decomposing fungus – Trichoderma spp
Nutrients:
• Organic carbon
• Seaweed fermentation derivatives
• Protein hydrolysates of vegetative origin from enzyme hydrolysis
Vitamins:
• Ascorbic acid
• Thiamine
Beneficial microbes and natural biostimulants contained in AgriLife Rootambio® act as bio primers that help in:
• Deep proliferation of roots
• Robust vegetative growth
• Disease suppression
• Drought tolerance
Biofertilizers:
• Nitrogen fixing bacteria helps fix atmospheric nitrogen into the soil
• Phosphorus solubilizing bacteria helps better solubilization of fixed phosphorus
• Potassium mobilizing bacteria - Potash is an expensive element and usually gets leached out. The bacteria mobilize potassium and makes this available to the plant
• Mycorrhiza colonize in root system and extend hyphae beyond the root system. This extended root system reaches out into zones normally not accessed by the root. This ensures plants getting more phosphorus and other micro nutrients from ‘far away’ zones. Mycorrhiza offers unique bio stimulant properties that enhance tolerance to biotic and abiotic stress
PGPR:
• Silica solubilizing bacteria impart drought tolerance and promote robust plant growth
• Bacillus spp promotes a healthy root system and has a fungistatic action
• Trichoderma spp decomposes biomass in soil and has a fungistatic action
• Organic acids and enzymes secreted by the microbes help in mobilizing various fixed elements in the soil
Nutrients:
• Organic carbon offers a congenial micro environment, increased organic carbon for growth and development of beneficial microbes
• Seaweed fermentation derivatives contain cytokinins, auxins, gibberellins and betaines which aid in cell multiplication, cell elongation and vegetative growth
• Protein hydrolysates of vegetative origin derived from enzyme hydrolysis process contain useful amino acids that help in robust plant growth
Vitamins:
• Ascorbic acid and Thiamine help address abiotic stress management of crops
AgriLife Rootambio® is available in the following formulations:
The following table gives details on Application methods and doses for the different formulations
| Application type | Soluble powder formulation |
| Dose | 1-1.5g / L water |
| Seed dressing | Mix AgriLife Rootambio® in sufficient water to make a slurry and coat seeds. |
| Seedling / planting material dip | Dissolve 1.5 g / L of water and dip seedling / planting material in the solution such that it gets coated completely with the solution before transplanting to main field |
Seed Dressing: Mix AgriLife Rootambio® with crude sugar in sufficient water to make a slurry and coat seeds and dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds more than 24 hrs
Note:
Do not store AgriLife Rootambio® solution for more than 24 hours after mixing in water.
A fraction of the product may not be soluble in water. It is advisable to filter and discard undissolved residue and use filtered liquid for spraying or feeding through drip irrigation.
Compatible with biofertilizers, organic fertilizers, organic manures, biopesticides, vermicompost, sea kelp (seaweed fertilizer), protein hydrolysates, organic carbon and rock phosphate.
Soluble powder formulation: AgriLife Rootambio® can be dry coated on chemical fertilizer granules / prills before application. AgriLife Rootambio® can be mixed with conventional chemical fertilizers/ chemical pesticides as a tank mix and drench / spray immediately on soil / crops.
Note:
It is advisable to have a one-week gap if soil application pesticides are to be used after application of AgriLife Rootambio®.
Store in ambient temperature, not more than 35 degrees Celsius. Preferably store between 4 – 10-degree Celsius. Keep container closed after use. Store in a dry place.
AgriLife Rootambio® has a shelf life of 1 Year from the date of manufacturing.
AgriLife Rootambio® composition can be customized to meet local regulatory guidelines.
AgriLife Rootambio® is offered in India - as Phosphorus solubilizing bacteria – Biofertilizer under Fertilizer Control Order (FCO) of Govt of India with label claim of Bacillus megaterium with 5*10^7 CFU/g.
Can I spray AgriLife Rootambio® during day time or a hot day?
Yes. You can spray anytime. Organic carbon in the product facilitates priming of microbes for better germination and survival.
Can I mix AgriLife Rootambio® with chemical fungicides?
No. Do not mix chemical fungicides with AgriLife Rootambio®.
Are there any studies to show if particular species of soil microbes are more prevalent in the rhizosphere during vegetative growth, and some species more prevalent during generative growth? Is there a shift in the soil microbial community at different growth stages, and if this can be manipulated through the use of inoculums at targeted times or is it best to apply a diversity of species during early stage growth? This is for growing crops in containers in controlled environments, not open soil.
Soil microbiome has formed in 1000s of years and acclimatised micirobes keep growing under normal ecosystem. However, with industrial agricultural practices of last 7-8 decades, use of herbicides, other pesticides and fertilizers / Chemicals has impaired the soil microbiome. Therefore, natural selection which happened over 1000s of years - is disturbed. There is no specific sequencing of microbes from early stage of crops to final stages.
Now we wish to play safe for the introduction of inoculum in uncertain soil conditions. We have carefully selected endospore Forming Gram Positive Bacteria - mainly Bacilli. These microbes are multiplied in fermenter, centrifuged and spray dried to get concentrated spores. Drop down temperature from nozzle on top to bottom of spray drier is 180 degree C to 70-degree C. Thus, all weak spores will die and strong endospores are harvested.
The Spores bring concentrated - we can do judicious blend of many Bacilli and make a formulation that is highly stable across all field soil temperatures and soil moisture levels.
AgriLife Rootambio® contains Nitrogen Fixing Bacteria, Phosphorus Solubilizing Bacteria, Potash Mobilising Bacteria, Silica Solubilizing Bacteria, Plant Growth Promoting Rhizobacteria, Mycorrhiza and Nutrients for Bacteria (PreBiotics) like Humic / Fulvic substances and Seaweed fermentation derivatives plus Stress Busters like Ascorbic Acid and Thiamine
The above combination of bacteria and nutrients help in providing a near natural eco microbial ecosystem irrespective of it being a soil medium or a soil free / no soil medium
